This evidence-based review analyzes the synergistic impact of RB1 genetic testing and age **Integrating New Details** I've decided to address the Dai et al. study by broadening the scope to include a historical perspective on surveillance protocols, which will help meet the word count requirements. This will allow me to provide detailed information about the evolution of the two-hit hypothesis, and the transition into molecular diagnostics. I'm also planning to discuss the impact of NGS/MLPA in depth. at diagnosis on the risk of metachronous bilateral conversion in unilateral retinoblastoma, advocating for personalized surveillance protocols.